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Immunocytochemistry of anti-FLOT1/2 antibodies (in house transfection) . HEK293 cells co-transfected with mammalian-expression vectors encoding human FLOT1 and <t>FLOT2</t> using Lipofectamine 2000; double-stained with serum ( B , E ) and with commercial antibody against FLOT1 ( A , D ). MS patient’s IgG bind to co-transfected cells ( B ) and colocalize with FLOT1 ab ( C ); in contrast with the healthy control ( E ) that doesn’t show any reactivity against FLOT-1/2 antibodies ( F ).
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Immunocytochemistry of anti-FLOT1/2 antibodies (in house transfection) . HEK293 cells co-transfected with mammalian-expression vectors encoding human FLOT1 and <t>FLOT2</t> using Lipofectamine 2000; double-stained with serum ( B , E ) and with commercial antibody against FLOT1 ( A , D ). MS patient’s IgG bind to co-transfected cells ( B ) and colocalize with FLOT1 ab ( C ); in contrast with the healthy control ( E ) that doesn’t show any reactivity against FLOT-1/2 antibodies ( F ).
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Immunocytochemistry of anti-FLOT1/2 antibodies (in house transfection) . HEK293 cells co-transfected with mammalian-expression vectors encoding human FLOT1 and FLOT2 using Lipofectamine 2000; double-stained with serum ( B , E ) and with commercial antibody against FLOT1 ( A , D ). MS patient’s IgG bind to co-transfected cells ( B ) and colocalize with FLOT1 ab ( C ); in contrast with the healthy control ( E ) that doesn’t show any reactivity against FLOT-1/2 antibodies ( F ).

Journal: Brain Communications

Article Title: Antibodies against the flotillin-1/2 complex in patients with multiple sclerosis

doi: 10.1093/braincomms/fcad109

Figure Lengend Snippet: Immunocytochemistry of anti-FLOT1/2 antibodies (in house transfection) . HEK293 cells co-transfected with mammalian-expression vectors encoding human FLOT1 and FLOT2 using Lipofectamine 2000; double-stained with serum ( B , E ) and with commercial antibody against FLOT1 ( A , D ). MS patient’s IgG bind to co-transfected cells ( B ) and colocalize with FLOT1 ab ( C ); in contrast with the healthy control ( E ) that doesn’t show any reactivity against FLOT-1/2 antibodies ( F ).

Article Snippet: First, all the samples were analysed for IgG reactivity on commercial slides containing biochips with cells co-expressing FLOT1 and FLOT2 proteins (Euroimmun, Lübeck, Germany).

Techniques: Immunocytochemistry, Transfection, Expressing, Staining

Immunoadsorption of anti-FLOT1/2 antibodies . HEK293 cells co-transfected with mammalian-expression vectors encoding human FLOT1 and FLOT2 double-stained with serum immunoadsorbed with non-transfected cells ( A ), FLOT1-transfected cells ( D ), FLOT2-transfected cells ( G ) or FLOT1 and FLOT2 co-transfected cells ( J ); and with commercial antibody against FLOT1 ( B , E , K ) or FLOT2 ( H ). Reactivity against the FLOT-1/2 complex was lost after serum pre-adsorption with HEK cells co-expressing FLOT1 and FLOT2 ( L ), but not after pre-adsorption with cells transfected with FLOT1 ( F ) or FLOT2 ( I ) alone or with non-transfected HEK cells ( C ).

Journal: Brain Communications

Article Title: Antibodies against the flotillin-1/2 complex in patients with multiple sclerosis

doi: 10.1093/braincomms/fcad109

Figure Lengend Snippet: Immunoadsorption of anti-FLOT1/2 antibodies . HEK293 cells co-transfected with mammalian-expression vectors encoding human FLOT1 and FLOT2 double-stained with serum immunoadsorbed with non-transfected cells ( A ), FLOT1-transfected cells ( D ), FLOT2-transfected cells ( G ) or FLOT1 and FLOT2 co-transfected cells ( J ); and with commercial antibody against FLOT1 ( B , E , K ) or FLOT2 ( H ). Reactivity against the FLOT-1/2 complex was lost after serum pre-adsorption with HEK cells co-expressing FLOT1 and FLOT2 ( L ), but not after pre-adsorption with cells transfected with FLOT1 ( F ) or FLOT2 ( I ) alone or with non-transfected HEK cells ( C ).

Article Snippet: First, all the samples were analysed for IgG reactivity on commercial slides containing biochips with cells co-expressing FLOT1 and FLOT2 proteins (Euroimmun, Lübeck, Germany).

Techniques: Transfection, Expressing, Staining, Adsorption